Regulation of histone methylation
Ryan will be investigating the regulation of histone methylation in Saccharomyces cerevisiae. He will use mass spectrometric techniques to systematically characterise all yeast histone methyl ‘writers’ (methyltransferases) and ‘erasers’ (demethylases). More specifically, he aims to identify how phosphorylation events on these enzymes affect their catalytic activity, localisation, and interaction partners. By identifying the upstream kinases responsible for specific phosphorylation sites, this work will delineate the complete regulatory network of histone methylation in yeast.
Image source: Breitkreutz A, Choi H, Sharom JR, et al. 2010. A Global Protein Kinase and Phosphatase Interaction Network in Yeast. Science 328 (5981), 1043-1046. doi: 10.1126/science.1176495.