What is protein methylation and where is it found? What enzymes are responsible? Also, what is its function? We are addressing these questions with proteomics, mass spectrometry and two-hybrid-based techniques to build intracellular methylproteome networks.
We are studying the histone methylation system in yeast, and its regulation. An important question is how the eight methylation enzymes are phosphorylated, and are connected to the cell's kinases and signalling system.
Large-scale Studies of Protein-Protein Interaction and Structure
We are using cross-linking and mass spectrometry, to detect hundreds of protein-protein interactions and measures of structure in a single experiment. These interactions and structures are yielding powerful insights into complex phenotypes.