Past projects – proteomic analysis of protein methylation and its regulation by phosphorylation
How do post-translational modifications such as phosphorylation, acetylation and methylation affect each other? In his PhD, Daniel studied how protein methylation can be best detected, exploring the use of ion mobility mass spectrometry techniques with collaborator Prof. Alex Donald. He also comprehensively characterised a range of protein methyltransferases, showing that they all are heavily phosphorylated and that some are acetylated. This work strongly suggested that these enzymes are regulated by phosphorylation. His research work was published in Analytical Chemistry, the Journal of Molecular Biology, and in Proteomics. Daniel also wrote a range of excellent reviews, including work published in Trends in Biotechnology.