Daniela Smith

Project Detail

Post-translational modifications and the interactome

Large scale yeast two hybrid, and TAP-TAG affinity purification strategies have allowed robust analyses of the yeast interactome. However we do not yet have a holistic understanding of the dynamic nature of the interactome, particularly concerning the influences of post-translational modifications on protein-protein interactions. I am using cross-linking mass spectrometry to uncover PTM modulated interactions, on an interactome scale.