Dr Gene Hart-Smith

Project Detail

Methylation in protein interactions

I am using our existing knowledge of the S. cerevisiae intracellular methylation network to conduct an organism-wide survey of protein-protein interactions mediated by methylation. My  research makes use of an innovative mass spectrometry-based workflow, centred around a technique known as Size Exclusion Chromatography-Protein Correlation Profiling-Stable Isotope Labelling by Amino acids in Cell culture (SEC-PCP-SILAC), to monitor the role of methylation in protein complex formation.

Publications
http://www.mcponline.org/content/15/3/989.long
http://pubs.acs.org/doi/abs/10.1021/pr401251k

Google scholar profile